5T7U

Crystal structure of galectin-8N in complex with Glycerol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-15
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	47.400, 50.300, 69.390
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.730 - 1.580
R-factor	0.11938
Rwork	0.118
R-free	0.14657
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ap5
RMSD bond length	0.011
RMSD bond angle	1.546
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall
Low resolution limit [Å]	40.730
High resolution limit [Å]	1.580
Rmerge	0.046
Number of reflections	23530
<I/σ(I)>	24.3
Completeness [%]	100.0
Redundancy	7.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	10 mM sodium phosphate, 137 mM sodium chloride, 2.7 mM potassium chloride, 1.8 mM potassium phosphate