5T7I
Crystal structure of galectin-8N in complex with LNnT
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-10-15 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.9537 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.170, 50.140, 69.860 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.730 - 2.000 |
R-factor | 0.19068 |
Rwork | 0.189 |
R-free | 0.21679 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ap5 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.393 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 40.730 |
High resolution limit [Å] | 2.000 |
Rmerge | 0.057 |
Number of reflections | 11695 |
<I/σ(I)> | 26 |
Completeness [%] | 99.6 |
Redundancy | 8.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 10 mM sodium phosphate, 137 mM sodium chloride, 2.7 mM potassium chloride, 1.8 mM potassium phosphate |