5T5V
LIPOXYGENASE-1 (SOYBEAN) AT 293K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 300 |
| Detector technology | CCD |
| Collection date | 2012-12-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.8266 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 91.572, 92.754, 100.919 |
| Unit cell angles | 90.00, 93.76, 90.00 |
Refinement procedure
| Resolution | 91.380 - 1.800 |
| R-factor | 0.135 |
| Rwork | 0.134 |
| R-free | 0.17100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3pzw |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.030 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10PRE_2104) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 92.754 | 92.754 | 1.900 |
| High resolution limit [Å] | 1.800 | 5.690 | 1.800 |
| Rmerge | 0.027 | 0.886 | |
| Number of reflections | 154694 | ||
| <I/σ(I)> | 8 | 18.5 | 0.8 |
| Completeness [%] | 100 | 98.8 | |
| Redundancy | 4.6 | 4.7 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 300 | PEG-3350, Sodium Acetate |
