5T5S

A fragment of a human tRNA synthetase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-11-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.987
Spacegroup name	C 2 2 21
Unit cell lengths	90.829, 136.141, 59.077
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.491 - 2.202
R-factor	0.2108
Rwork	0.208
R-free	0.25840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5t76
RMSD bond length	0.008
RMSD bond angle	1.023
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((dev_2306: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.557	2.280
High resolution limit [Å]	2.202	2.202
Rmerge		0.109
Number of reflections	16637
<I/σ(I)>	11.5
Completeness [%]	98.6
Redundancy	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	290	0.2 M ammonium acetate, 0.1 M Tris, pH 8.5, 25% w/v PEG3350