5T5F
Neisseria meningitidis factor H binding protein in complex with monoclonal antibody JAR5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | OTHER |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-29 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 65.370, 146.490, 218.220 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.700 - 2.980 |
| R-factor | 0.225 |
| Rwork | 0.223 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2w80 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.230 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.240 | 3.160 |
| High resolution limit [Å] | 2.980 | 2.980 |
| Rmerge | 1.354 | |
| Number of reflections | 21885 | |
| <I/σ(I)> | 5.5 | 1.3 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 5.6 | 5 |
| CC(1/2) | 0.970 | 0.429 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293.15 | 20% Polyethylene glycol (PEG) 6000 0.1 M tri-Sodium citrate, pH 5.0 |
