5T59

Structure of the MIND Complex Shows a Regulatory Focus of Yeast Kinetochore Assembly

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-27
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979200
Spacegroup name	P 21 21 21
Unit cell lengths	75.400, 91.700, 95.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	66.168 - 2.405
R-factor	0.2513
Rwork	0.249
R-free	0.29050
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.618
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	66.170
High resolution limit [Å]	2.405
Rmerge	0.073
Number of reflections	16798
<I/σ(I)>	11.9
Completeness [%]	56.5
Redundancy	3.6
CC(1/2)	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M CHES, pH 9.0, 18-30 % PEG 3000