5T58
Structure of the MIND Complex Shows a Regulatory Focus of Yeast Kinetochore Assembly
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-09-11 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97910 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.750, 172.570, 59.480 |
| Unit cell angles | 90.00, 110.46, 90.00 |
Refinement procedure
| Resolution | 86.285 - 3.213 |
| R-factor | 0.3225 |
| Rwork | 0.320 |
| R-free | 0.36200 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.650 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.290 | 3.330 |
| High resolution limit [Å] | 3.210 | 3.210 |
| Rmerge | 0.207 | |
| Number of reflections | 13484 | |
| <I/σ(I)> | 9.5 | |
| Completeness [%] | 74.4 | |
| Redundancy | 11.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M Tris-Cl, pH 8.0, 18-22% PEG 550 MME, 0.5-4.5 % poly-gamma-glutamic acid low molecular weight (PGA-LM), 0.2 M potassium thiocyanate, 0.2 M potassium bromide |
