5T51
Structure of the MIND Complex Shows a Regulatory Focus of Yeast Kinetochore Assembly
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-12 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 62 |
| Unit cell lengths | 82.630, 82.630, 62.470 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.780 - 2.201 |
| R-factor | 0.2123 |
| Rwork | 0.208 |
| R-free | 0.25310 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.443 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.780 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.201 |
| Rmerge | 0.119 | |
| Number of reflections | 12402 | |
| <I/σ(I)> | 9 | 1.5 |
| Completeness [%] | 97.0 | 100 |
| Redundancy | 6.5 | 5.5 |
| CC(1/2) | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M cacodylate, pH 6.5, 2.0 M ammonium sulfate, 0.2 M sodium chloride |
