5T4A
Crystal structure of BhGH81 in complex with laminaro-hexaose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-05-27 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.9783 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 80.910, 96.140, 104.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.840 - 2.100 |
| R-factor | 0.1599 |
| Rwork | 0.158 |
| R-free | 0.20190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5t49 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.448 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.840 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.102 | 0.313 |
| Number of reflections | 44790 | |
| <I/σ(I)> | 10.8 | 5.6 |
| Completeness [%] | 93.1 | 87 |
| Redundancy | 4.5 | 4.5 |
| CC(1/2) | 0.986 | 0.874 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 20% PEG 3350, 0.2 M sodium iodide |
