5T44

Crystal structure of Frizzled 7 CRD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	93
Detector technology	PIXEL
Collection date	2015-01-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	39.056, 62.846, 103.225
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.500 - 1.994
R-factor	0.2016
Rwork	0.199
R-free	0.25220
RMSD bond length	0.006
RMSD bond angle	0.944
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	36.500
High resolution limit [Å]	1.994
Number of reflections	35844
<I/σ(I)>	10.3
Completeness [%]	99.7
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.9	277	0.1M MES at pH6.9, 12% 1-propanol, 10% PEG 500MME