5T3W

Marburg virus VP30 bound to nucleoprotein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-12
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.750200
Spacegroup name	P 31 2 1
Unit cell lengths	106.143, 106.143, 375.573
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	125.190 - 3.250
R-factor	0.21401
Rwork	0.213
R-free	0.24082
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5dvw
RMSD bond length	0.007
RMSD bond angle	1.142
Data reduction software	XDS
Data scaling software	XDS (Oct 15, 2015)
Phasing software	PHASER (2.6.1)
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	125.191	3.380
High resolution limit [Å]	3.250	3.250
Rmerge	0.110	2.960
Number of reflections	39861
<I/σ(I)>	15.9	1.1
Completeness [%]	100.0	100
Redundancy	19.1	19.5
CC(1/2)	1.000	0.610

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	298	20% isopropanol, 18% PEG 4000, 0.1M sodium citrate pH 5.6 using 0.2 uL 24.7 mg/mL protein mixed with 0.2 uL mother liquor