5T3S
HIV gp140 trimer MD39-10MUTA in complex with Fabs PGT124 and 35022
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.03322 |
| Spacegroup name | P 63 |
| Unit cell lengths | 127.915, 127.915, 313.887 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.010 - 4.500 |
| R-factor | 0.27158 |
| Rwork | 0.270 |
| R-free | 0.30877 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cez |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.513 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.010 | 4.580 |
| High resolution limit [Å] | 4.500 | 4.500 |
| Rmerge | 0.213 | 1.000 |
| Number of reflections | 17067 | |
| <I/σ(I)> | 4.75 | 1.1 |
| Completeness [%] | 98.4 | 98.9 |
| Redundancy | 4.3 | 4.4 |
| CC(1/2) | 0.760 | 0.630 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.1 | 277 | 5% Peg6000 0.1M citric acid |
