5T39
Crystal Structure of the N-terminal domain of EvdMO1 in the presence of SAH and D-fucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-06-03 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.222, 41.270, 58.504 |
| Unit cell angles | 90.00, 98.58, 90.00 |
Refinement procedure
| Resolution | 22.472 - 1.100 |
| R-factor | 0.1378 |
| Rwork | 0.137 |
| R-free | 0.15390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mgg |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.009 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.120 |
| High resolution limit [Å] | 1.100 | 2.990 | 1.100 |
| Rmerge | 0.060 | 0.042 | 0.434 |
| Rmeas | 0.068 | ||
| Rpim | 0.031 | ||
| Total number of observations | 421834 | ||
| Number of reflections | 94601 | ||
| <I/σ(I)> | 9.2 | ||
| Completeness [%] | 98.5 | 96.4 | 95.3 |
| Redundancy | 4.5 | 4.3 | 3.7 |
| CC(1/2) | 0.996 | 0.868 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 40% Tacsimate, pH 7.4 |
