5T39

Crystal Structure of the N-terminal domain of EvdMO1 in the presence of SAH and D-fucose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2016-06-03
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 1 21 1
Unit cell lengths	50.222, 41.270, 58.504
Unit cell angles	90.00, 98.58, 90.00

Refinement procedure

Resolution	22.472 - 1.100
R-factor	0.1378
Rwork	0.137
R-free	0.15390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3mgg
RMSD bond length	0.006
RMSD bond angle	1.009
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.120
High resolution limit [Å]	1.100	2.990	1.100
Rmerge	0.060	0.042	0.434
Rmeas	0.068
Rpim	0.031
Total number of observations	421834
Number of reflections	94601
<I/σ(I)>	9.2
Completeness [%]	98.5	96.4	95.3
Redundancy	4.5	4.3	3.7
CC(1/2)		0.996	0.868

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	293	40% Tacsimate, pH 7.4