5T2Q
Unliganded Human HVEM at 1.9A in P 1 21 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.975 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.190, 47.510, 67.290 |
| Unit cell angles | 90.00, 106.69, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.25373 |
| Rwork | 0.252 |
| R-free | 0.28751 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2aw2 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.062 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.980 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.105 | |
| Number of reflections | 23026 | |
| <I/σ(I)> | 16.2 | 1.062 |
| Completeness [%] | 99.3 | 94.7 |
| Redundancy | 7.6 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 0.15 M CaCl2, 15% (w/v) PEG 3300, 30% (w/v) PEG 8K, 5% (v/v) Glycerol |
