5T29
Crystal structure of 10E8 Fab light chain mutant3, against the MPER region of the HIV-1 Env, in complex with the MPER epitope scaffold T117v2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2015-06-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97950 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 110.879, 66.972, 84.259 |
| Unit cell angles | 90.00, 101.46, 90.00 |
Refinement procedure
| Resolution | 36.560 - 2.030 |
| R-factor | 0.176 |
| Rwork | 0.174 |
| R-free | 0.21300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4g6f 3lf6 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.007 |
| Data reduction software | HKL-2000 (2.3.8) |
| Data scaling software | HKL-2000 (2.3.8) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.560 | 2.070 |
| High resolution limit [Å] | 2.025 | 2.030 |
| Rmerge | 0.535 | |
| Number of reflections | 36193 | |
| <I/σ(I)> | 7.6 | 2.5 |
| Completeness [%] | 92.1 | 87 |
| Redundancy | 2.5 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 295.15 | 0.1 M PO4-citrate pH 4.2, 40% PEG 600 |
