5T1G
chromo shadow domain of CBX1 in complex with a histone peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-12-12 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9791827 |
Spacegroup name | F 2 2 2 |
Unit cell lengths | 69.657, 76.887, 78.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.070 - 1.900 |
R-factor | 0.2156 |
Rwork | 0.213 |
R-free | 0.24310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fmm |
RMSD bond length | 0.012 |
RMSD bond angle | 1.480 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.27) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 43.070 | 43.070 | 1.840 |
High resolution limit [Å] | 1.800 | 9.000 | 1.800 |
Rmerge | 0.075 | 0.028 | 1.478 |
Rmeas | 0.081 | 0.031 | 1.596 |
Rpim | 0.030 | 0.013 | 0.596 |
Total number of observations | 71509 | 525 | 3905 |
Number of reflections | 9891 | ||
<I/σ(I)> | 16.9 | 56.9 | 1.3 |
Completeness [%] | 99.9 | 97 | 99.4 |
Redundancy | 7.2 | 5.6 | 6.9 |
CC(1/2) | 0.999 | 0.997 | 0.788 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 1.5 M sodium citrate, 0.1 M HEPES |