5T11
PelC L103M dodecamer from Paraburkholderia phytofirmans, space group C2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-01-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 141.279, 167.197, 94.182 |
| Unit cell angles | 90.00, 104.15, 90.00 |
Refinement procedure
| Resolution | 49.316 - 2.104 |
| R-factor | 0.1939 |
| Rwork | 0.193 |
| R-free | 0.23410 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.788 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.095 | 0.720 |
| Number of reflections | 122137 | |
| <I/σ(I)> | 27 | |
| Completeness [%] | 99.2 | 92.7 |
| Redundancy | 7.6 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 1.45 M ammonium sulfate, 0.1 M MES pH 5.7 |
