5T10
PelC dodecamer from Paraburkholderia phytofirmans, space group P6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 6 |
| Unit cell lengths | 118.113, 118.113, 85.688 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.626 - 2.499 |
| R-factor | 0.1895 |
| Rwork | 0.185 |
| R-free | 0.23550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5t11 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.058 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.499 | 2.499 |
| Rmerge | 0.110 | 0.759 |
| Number of reflections | 23687 | |
| <I/σ(I)> | 28.5 | |
| Completeness [%] | 98.0 | 87.2 |
| Redundancy | 21.1 | 15.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 1 M NaH2PO4/KHPO4 pH 5.6 |
