5T09

The structure of the type III effector HopBA1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-02
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0000
Spacegroup name	P 31 2 1
Unit cell lengths	64.865, 64.865, 96.380
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.127 - 2.013
R-factor	0.2016
Rwork	0.200
R-free	0.23240
RMSD bond length	0.003
RMSD bond angle	0.505
Data reduction software	HKL-2000 (2.0.0)
Data scaling software	HKL-2000 (2.0.0)
Phasing software	CRANK (1.3.0)
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall
Low resolution limit [Å]	32.130
High resolution limit [Å]	2.010
Number of reflections	16048
<I/σ(I)>	49.9
Completeness [%]	99.9
Redundancy	9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	6uL hanging vapor diffusion drops over 0.5M K2SO4, 0.1M HEPES pH 7.0, and 30% v/v PEG 400. Protein concentration 10.7 ug/mL. 1:1 ratio protein:mother liquor