5SZY
Novel Structural Insights into GDP-Mediated Regulation of Acyl-CoA Thioesterases
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-06-17 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9537 |
Spacegroup name | P 21 3 |
Unit cell lengths | 152.800, 152.800, 152.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.015 - 2.000 |
R-factor | 0.1993 |
Rwork | 0.199 |
R-free | 0.21240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ien |
RMSD bond length | 0.008 |
RMSD bond angle | 1.430 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.020 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.020 | |
Number of reflections | 80089 | |
<I/σ(I)> | 23.55 | 7.64 |
Completeness [%] | 100.0 | 100 |
Redundancy | 12 | 12.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 296 | 100 mM Tris pH 8.5 and 2 M ammonium phosphate |