5SZX
Epstein-Barr virus Zta DNA binding domain homodimer in complex with methylated DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-02-21 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 95.549, 26.732, 99.673 |
| Unit cell angles | 90.00, 97.25, 90.00 |
Refinement procedure
| Resolution | 32.959 - 2.251 |
| R-factor | 0.2608 |
| Rwork | 0.260 |
| R-free | 0.28400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2c9l |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.833 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.086 | 0.885 |
| Number of reflections | 10789 | |
| <I/σ(I)> | 16.8 | 1.09 |
| Completeness [%] | 88.7 | 48.9 |
| Redundancy | 7.2 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2 M NaH2PO4-H2O 20% PEG3350 |
