5SZN
Protocadherin gamma A9 extracellular cadherin domains 1-5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 191.680, 107.610, 49.870 |
| Unit cell angles | 90.00, 97.14, 90.00 |
Refinement procedure
| Resolution | 19.996 - 2.944 |
| R-factor | 0.2436 |
| Rwork | 0.241 |
| R-free | 0.29540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PCDHGA8 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.667 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.120 |
| High resolution limit [Å] | 2.940 | 2.940 |
| Rmerge | 0.229 | 3.156 |
| Number of reflections | 13469 | |
| <I/σ(I)> | 4.5 | 0.6 |
| Completeness [%] | 98.9 | 98.8 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 6.5 | 295 | 10% (w/v) PEG4000, 20% (v/v) glycerol, 30 mM magnesium chloride, 30 mM calcium chloride, 0.1 M Morpheus Buffer System 1 (Mes/Imidazole buffer; Molecular Dimensions) pH 6.5 |
