5SZG

Structure of the bMERB domain of Mical-3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2009-12-11
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.978956
Spacegroup name	P 21 21 21
Unit cell lengths	51.858, 78.848, 95.557
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.780 - 2.700
R-factor	0.25245
Rwork	0.251
R-free	0.28208
Structure solution method	SAD
RMSD bond length	0.016
RMSD bond angle	1.809
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.800	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.138	1.150
Number of reflections	20544
<I/σ(I)>	16.8	3.6
Completeness [%]	99.1	98.5
Redundancy	13.3	13.6
CC(1/2)		0.870

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M Tris pH 7.0 50 % PEG 200