5SZE

Crystal structure of Aquifex aeolicus Hfq-RNA complex at 1.5A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-06-20
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9195
Spacegroup name	P 6
Unit cell lengths	66.190, 66.190, 34.210
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.210 - 1.500
R-factor	0.1457
Rwork	0.144
R-free	0.17200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1u1s
RMSD bond length	0.005
RMSD bond angle	0.754
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10-2155_1309: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	34.210
High resolution limit [Å]	1.500
Number of reflections	13177
<I/σ(I)>	12.3
Completeness [%]	94.9
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	sodium cacodylate, PEG 8000, MPD, guanidinium