5SZC

Structure of human Dpf3 double-PHD domain bound to histone H3 tail peptide with monomethylated K4 and acetylated K14

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 43 21 2
Unit cell lengths	39.321, 39.321, 147.571
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.996 - 1.193
R-factor	0.1489
Rwork	0.147
R-free	0.16690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB 5SZB
RMSD bond length	0.009
RMSD bond angle	1.122
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.220	1.220
High resolution limit [Å]	1.200	1.200
Rmerge	0.056	0.636
Number of reflections	38068
<I/σ(I)>	29.5	3.8
Completeness [%]	99.8	97.5
Redundancy	12.2	10.3
CC(1/2)	0.999	0.934

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	1.2 M tri-sodium citrate, 0.1 M HEPES pH 7.5, 4% MPD