5SZB
Structure of human Dpf3 double-PHD domain bound to histone H3 tail peptide with acetylated K14
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-06-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 39.321, 39.321, 147.571 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.996 - 1.200 |
R-factor | 0.1394 |
Rwork | 0.138 |
R-free | 0.15540 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.390 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.610 | 1.220 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmerge | 0.050 | 0.260 |
Number of reflections | 38299 | |
<I/σ(I)> | 38.3 | 8.2 |
Completeness [%] | 99.9 | 98.4 |
Redundancy | 12.3 | 10.1 |
CC(1/2) | 0.999 | 0.978 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 1.2 M tri-sodium citrate, 0.1 M HEPES pH 7.5, 4% MPD |