5SXO

1.35 angstrom resolution crystal structure of beta-ketoacyl-ACP synthase II (FabF) from Listeria monocytogenes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-02-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 43 21 2
Unit cell lengths	73.750, 73.750, 170.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.517 - 1.350
R-factor	0.1488
Rwork	0.148
R-free	0.16530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3o04
RMSD bond length	0.003
RMSD bond angle	0.638
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((dev_2467: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.520	1.370
High resolution limit [Å]	1.350	1.350
Number of reflections	103454
<I/σ(I)>	8.9	2.6
Completeness [%]	99.7	100
Redundancy	4.8	3.7
CC(1/2)	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296.15	0.2 M potassium sodium tartrate tetrahydrate, 0.1 M sodium citrate tribasic dihydrate pH 5.6, 2 M ammonium sulphate, 22.5% glycerol