5SXL

Structure of EspG3 chaperone from the type VII (ESX-3) secretion system, space group P3221

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2012-12-15
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00
Spacegroup name	P 32 2 1
Unit cell lengths	59.150, 59.150, 183.100
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.237 - 2.460
R-factor	0.2083
Rwork	0.206
R-free	0.24700
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.494
Data reduction software	XDS (VERSION September 26, 2012)
Data scaling software	XSCALE (VERSION May 1, 2016 BUILT=20160617)
Phasing software	SHARP (2.8)
Refinement software	PHENIX (dev_2481)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.240	39.240	2.520
High resolution limit [Å]	2.460	11.000	2.460
Rmerge	0.075	0.033	0.882
Number of reflections	13995
<I/σ(I)>	12.18	31.78	1.62
Completeness [%]	98.6	89.2	99.8
Redundancy	5.4		5.5
CC(1/2)	0.998	0.999	0.793

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	295	0.1 M HEPES, pH 7.4, 1.0 M lithium chloride, 10% PEG6000