5SXA

Crystal Structure of PI3Kalpha in complex with fragment 10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-10-17
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	115.225, 117.367, 152.057
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	92.910 - 3.350
R-factor	0.2245
Rwork	0.222
R-free	0.27450
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	4ovu
RMSD bond length	0.009
RMSD bond angle	1.332
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	92.910	50.000	3.470
High resolution limit [Å]	3.350	7.210	3.350
Rmerge	0.104	0.039	0.862
Number of reflections	30528
<I/σ(I)>	8.4
Completeness [%]	100.0	100	100
Redundancy	7.3	6.6	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	NaFormate