5SWB
Crystal structure of N-glycan transport solute binding protein (NgtS) from Streptococcus pneumoniae in complex with Man5GlcNAc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-11 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.98005 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 104.180, 110.600, 105.840 |
| Unit cell angles | 90.00, 109.52, 90.00 |
Refinement procedure
| Resolution | 99.760 - 1.730 |
| R-factor | 0.182 |
| Rwork | 0.180 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5suo |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 99.760 |
| High resolution limit [Å] | 1.730 |
| Number of reflections | 234916 |
| <I/σ(I)> | 25.4 |
| Completeness [%] | 99.9 |
| Redundancy | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 291 | 30 % PEG 400, 0.1 M cadmium chloride hydrate, 0.1 M sodium acetate trihydrate |
