5SW9

The structure of the PP2A B56 subunit RepoMan complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-09
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	53.598, 108.959, 120.065
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.207 - 2.846
R-factor	0.2013
Rwork	0.198
R-free	0.22710
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5k6s
RMSD bond length	0.002
RMSD bond angle	0.654
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.207	3.000
High resolution limit [Å]	2.846	2.850
Rmerge	0.097	0.885
Number of reflections	16968
<I/σ(I)>	13	2
Completeness [%]	99.1	97.3
Redundancy	5.5	5.6
CC(1/2)		0.686

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.75	298	8% PEG 8K, 0.8 M HEPES, 0.8 M LiCl