Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM16 |
| Synchrotron site | ESRF |
| Beamline | BM16 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.00801 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 100.343, 114.851, 174.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.050 |
| R-factor | 0.1505 |
| Rwork | 0.149 |
| R-free | 0.18430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1MWV |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.141 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 96.000 | 2.100 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Number of reflections | 119529 | |
| <I/σ(I)> | 13 | 2.2 |
| Completeness [%] | 99.3 | 94.4 |
| Redundancy | 4.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 16-20% MPD, 0.1 M sodium citrate pH 7.6 |
