5SW0

Thaumatin Structure at pH 4.0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2015-12-06
Detector	RIGAKU RAXIS V
Wavelength(s)	0.8
Spacegroup name	P 21 21 21
Unit cell lengths	51.641, 52.071, 70.532
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.425 - 1.269
R-factor	0.1436
Rwork	0.142
R-free	0.16580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3vhg
RMSD bond length	0.006
RMSD bond angle	1.089
Data scaling software	HKL-2000
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.290
High resolution limit [Å]	1.269	1.269
Rmerge	0.054	0.499
Number of reflections	50840
<I/σ(I)>	64.11	5.61
Completeness [%]	100.0	100
Redundancy	6.2	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	293	PEG8000, 50 mM sodium citrate