5SVU
Structure and kinetics of the LOV domain of ZEITLUPE determine its circadian function in Arabidopsis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2013-03-24 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.91730 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 85.351, 85.351, 200.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.506 - 2.601 |
| R-factor | 0.1676 |
| Rwork | 0.164 |
| R-free | 0.23170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3d72 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.608 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.075 | 0.276 |
| Number of reflections | 26153 | |
| <I/σ(I)> | 55.4 | 7.8 |
| Completeness [%] | 97.9 | 86.8 |
| Redundancy | 10 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M Tris pH 8.5, 0.2 M Magnesium Chloride hexahydrate, 30% w/v PEG 4k |
