5SVG
Structure and kinetics of the LOV domain of ZEITLUPE determine its circadian function in Arabidopsis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2013-03-24 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | .979 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 84.969, 84.969, 199.507 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.076 - 2.500 |
| R-factor | 0.1792 |
| Rwork | 0.176 |
| R-free | 0.23780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3d72 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.579 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.076 | 0.279 |
| Number of reflections | 28780 | |
| <I/σ(I)> | 35 | 11.1 |
| Completeness [%] | 97.1 | 100 |
| Redundancy | 9 | 9.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M Tris pH 8.5, 0.1 M Magnesium Chloride hexahydrate, 28% w/v PEG 4k |
