5SV8
Crystal Structure of the catalytic nucleophile and surface cysteine mutant of VvEG16 in complex with a xyloglucan oligosaccharide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-09 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.249, 51.867, 82.609 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.487 - 1.588 |
| R-factor | 0.1538 |
| Rwork | 0.152 |
| R-free | 0.17880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dze |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.540 | 1.620 |
| High resolution limit [Å] | 1.588 | 1.588 |
| Rmerge | 0.190 | 0.699 |
| Number of reflections | 27417 | |
| <I/σ(I)> | 12.2 | |
| Completeness [%] | 99.5 | 91.2 |
| Redundancy | 6.2 | 5.5 |
| CC(1/2) | 0.990 | 0.772 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 277 | 5 mM DTT, 5 mM XXXG, 1 mM EDTA, 0.1 M Bicine, 30% (w/v) PEG 6000 |
