5SV6
Crystal structure of MxaJ from Methlophaga aminisulfidivorans MPT
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-12-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 37.127, 63.761, 99.246 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.728 - 1.920 |
R-factor | 0.1841 |
Rwork | 0.178 |
R-free | 0.23890 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.040 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.950 |
High resolution limit [Å] | 1.920 | 1.920 |
Rmerge | 0.071 | 0.254 |
Number of reflections | 18684 | |
<I/σ(I)> | 0.69 | 16.049 |
Completeness [%] | 99.9 | 100 |
Redundancy | 13.9 | 13.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.2M sodium bromide, 20%(w/v) PEG 3350, 10%(w/v) glycerol, 0.1M Tris HCl |