5SV5
1.0 Angstrom Crystal Structure of pre-Peptidase C-terminal Domain of Collagenase from Bacillus anthracis.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-08-02 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.533, 37.764, 42.810 |
| Unit cell angles | 90.00, 106.95, 90.00 |
Refinement procedure
| Resolution | 29.820 - 1.000 |
| R-factor | 0.13335 |
| Rwork | 0.133 |
| R-free | 0.14813 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.453 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.020 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.080 | 0.726 |
| Number of reflections | 55101 | |
| <I/σ(I)> | 44.5 | 2.7 |
| Completeness [%] | 99.5 | 97.7 |
| Redundancy | 6.8 | 4.3 |
| CC(1/2) | 0.818 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 292 | Protein: 16.0 mg/ml, 0.5M Sodium chloride, 0.01M Tris-HCL (pH 8.3); Screen: Classics II (C9), 1.1M Sodium malonate, 0.1M HEPES (pH 7.0), 0.5% (v/v) Jeffamine ED-2001; Cryo: 4M Sodium formate |
