5SV3
RTA1-33/44-198 (RVEC) bound to Single Domain Antibody A3C8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR-H |
| Temperature [K] | 150 |
| Detector technology | CCD |
| Collection date | 2015-09-11 |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 117.759, 130.432, 35.876 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 65.220 - 2.730 |
| R-factor | 0.22838 |
| Rwork | 0.226 |
| R-free | 0.26670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lc9 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.172 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.220 | 2.830 |
| High resolution limit [Å] | 2.730 | 2.730 |
| Rmerge | 0.454 | |
| Number of reflections | 15475 | |
| <I/σ(I)> | 11.91 | 4.52 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 13.97 | 14.19 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 290 | 0.1 M HEPES pH 7.0, 1.6 M Ammonium Sulfate |
