5SV1
Structure of the ExbB/ExbD complex from E. coli at pH 4.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-13 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.007 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 104.942, 196.748, 210.599 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.000 - 3.500 |
| R-factor | 0.2561 |
| Rwork | 0.255 |
| R-free | 0.29960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5sv0 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.965 |
| Data reduction software | DENZO (HKL2000) |
| Data scaling software | SCALEPACK (HKL2000) |
| Phasing software | PHASER (PHENIX) |
| Refinement software | PHENIX ((1.10_2142: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.000 | 3.630 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 1.000 | |
| Number of reflections | 55966 | |
| <I/σ(I)> | 8.9 | 1.4 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 5.2 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 288 | 100 mM Na-acetate, pH 4.5, 100 mM MgCl2, and 25% PEG 400 |
