5SUY

Domain-swapped dimer of human Dishevelled2 DEP domain: monoclinic crystal form crystallised from dimeric fraction

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-17
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.928190
Spacegroup name	P 1 21 1
Unit cell lengths	61.671, 60.174, 66.128
Unit cell angles	90.00, 117.62, 90.00

Refinement procedure

Resolution	41.980 - 1.880
R-factor	0.18641
Rwork	0.185
R-free	0.21702
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	unpublished model
RMSD bond length	0.015
RMSD bond angle	1.591
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.980	1.960
High resolution limit [Å]	1.880	1.880
Rmerge	0.085	0.963
Number of reflections	34407
<I/σ(I)>	13.1	1.8
Completeness [%]	99.0
Redundancy	6.9	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M MES 0.1 M NaCl 0.1 M Li2SO4 22-26% PEG400