5SUH

The structure of double ringed trimeric shell protein MSM0271 from the RMM microcompartment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-10
Detector	RAYONIX MX-300
Wavelength(s)	0.97949
Spacegroup name	P 63 2 2
Unit cell lengths	139.890, 139.890, 150.120
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.139 - 2.700
R-factor	0.1755
Rwork	0.173
R-free	0.22430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	MSM0275
RMSD bond length	0.010
RMSD bond angle	1.278
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.200	2.770
High resolution limit [Å]	2.700	2.700
Number of reflections	24387
<I/σ(I)>	19.2	2.1
Completeness [%]	99.9	100
Redundancy	9.6	11.1
CC(1/2)	0.997	0.752

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	10 mg/mL protein, 0.8 M Na/K tartrate, 0.1 M Tris, 0.5% (w/v) PEG 2000MME