5SJH

Crystal Structure of human phosphodiesterase 10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2007-05-16
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.00000
Spacegroup name	H 3
Unit cell lengths	136.308, 136.308, 235.947
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.720 - 2.100
R-factor	0.1787
Rwork	0.177
R-free	0.20360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	inhouse model
RMSD bond length	0.008
RMSD bond angle	0.870
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	BUSTER (2.11.7 (19-MAR-2020))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	41.720	50.000	2.070
High resolution limit [Å]	2.000	4.310	2.000
Rmerge	0.157	0.112
Total number of observations	3719
Number of reflections	3675	647	75
<I/σ(I)>	5.3
Completeness [%]	1.7	3	0.3
Redundancy	1	1	1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	295	5-20 mg/mL protein in 25mM HEPES/NaOH pH7.5, 150mM NaCl, 50mM BME mixed 1:1 with reservoir 0.1M HEPES/NaOH pH7.5, 30% PEG550MME, 50mM MgCl2