5RZN
EPB41L3 PanDDA analysis group deposition -- Crystal Structure of the FERM domain of human EPB41L3 in complex with Z1745658474
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-08-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.91589 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.560, 77.920, 99.730 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.040 - 1.830 |
| R-factor | 0.2107 |
| Rwork | 0.209 |
| R-free | 0.25160 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 6ibe |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.468 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 42.000 | 42.000 | 1.880 |
| High resolution limit [Å] | 1.830 | 8.180 | 1.830 |
| Rmerge | 0.105 | 0.026 | 1.659 |
| Rmeas | 0.114 | 0.028 | 1.800 |
| Rpim | 0.044 | 0.011 | 0.692 |
| Total number of observations | 175823 | 2100 | 12880 |
| Number of reflections | 27314 | ||
| <I/σ(I)> | 11.8 | 41.2 | 1.2 |
| Completeness [%] | 100.0 | 99.6 | 100 |
| Redundancy | 6.4 | 5.6 | 6.6 |
| CC(1/2) | 0.998 | 0.999 | 0.512 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 0.075M ammonium nitrate, 0.1M MES pH 6.0, 6.5% PEG 2000, 6.5% PEG 3350, 6.5% PEG 4000, 6.5% PEG 5000, 10% ethylene glycol |
