5RYU
EPB41L3 PanDDA analysis group deposition -- Crystal Structure of the FERM domain of human EPB41L3 in complex with Z1545196403
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.91587 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.445, 77.283, 99.439 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.020 - 1.630 |
| R-factor | 0.2106 |
| Rwork | 0.209 |
| R-free | 0.24720 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 6ibe |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.478 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 77.290 | 77.290 | 1.670 |
| High resolution limit [Å] | 1.630 | 7.290 | 1.630 |
| Rmerge | 0.089 | 0.037 | 1.364 |
| Rmeas | 0.097 | 0.041 | 1.525 |
| Rpim | 0.039 | 0.017 | 0.670 |
| Total number of observations | 233286 | 2839 | 14084 |
| Number of reflections | 37872 | ||
| <I/σ(I)> | 9.8 | 31.1 | 1 |
| Completeness [%] | 100.0 | 99.9 | 99.8 |
| Redundancy | 6.2 | 5.5 | 5.1 |
| CC(1/2) | 0.998 | 0.996 | 0.486 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 0.075M ammonium nitrate, 0.1M MES pH 6.0, 6.5% PEG 2000, 6.5% PEG 3350, 6.5% PEG 4000, 6.5% PEG 5000, 10% ethylene glycol |
