5ROB
PanDDA analysis group deposition of ground-state model of SARS-CoV-2 helicase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.91256 |
| Spacegroup name | P 1 |
| Unit cell lengths | 59.242, 70.282, 84.616 |
| Unit cell angles | 102.40, 95.71, 112.84 |
Refinement procedure
| Resolution | 80.920 - 1.870 |
| R-factor | 0.2205 |
| Rwork | 0.219 |
| R-free | 0.25380 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 6zsl |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.491 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 80.920 |
| High resolution limit [Å] | 1.870 |
| Number of reflections | 95763 |
| <I/σ(I)> | 8.2 |
| Completeness [%] | 96.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 16 % Ethylene Glycol, 8 % PEG 8K, 0.05 M HEPES, 0.05 M MOPS, 0.03 M Sodium Nitrate, 0,03 M Sodium Phosphate, 0.03 M Ammonium Sulphate |
