5QUF
HumRadA1.2 soaked with napht-2,3-diol overnight at 20 mM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-02-16 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9794 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 69.071, 72.919, 40.401 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.401 - 1.350 |
R-factor | 0.1701 |
Rwork | 0.169 |
R-free | 0.19570 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.401 | 40.401 | 1.430 |
High resolution limit [Å] | 1.350 | 4.030 | 1.350 |
Rmerge | 0.089 | 0.050 | 1.147 |
Rmeas | 0.096 | 0.055 | 1.267 |
Total number of observations | 296891 | ||
Number of reflections | 42489 | 1854 | 4901 |
<I/σ(I)> | 12.08 | 32.68 | 1.35 |
Completeness [%] | 93.0 | 99.3 | 67.1 |
Redundancy | 6.987 | 6.643 | 5.369 |
CC(1/2) | 0.998 | 0.997 | 0.505 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 50 mM Na/K phospate, 5% PEG1000H |