5QTB
T. brucei FPPS in complex with CID 303798
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-03 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.999930 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 60.961, 60.961, 340.711 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 52.794 - 1.965 |
R-factor | 0.231 |
Rwork | 0.229 |
R-free | 0.27180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | pdbid 4ryp |
Data reduction software | XDS (20180808) |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER (2.8.2) |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.794 | 2.112 |
High resolution limit [Å] | 1.965 | 1.965 |
Rmerge | 0.139 | 2.454 |
Rmeas | 0.189 | 5.711 |
Rpim | 0.044 | 1.279 |
Total number of observations | 436762 | 71436 |
Number of reflections | 17981 | 3623 |
<I/σ(I)> | 17.8 | 0.7 |
Completeness [%] | 63.9 | 17 |
Redundancy | 18.2 | 18.9 |
CC(1/2) | 0.999 | 0.480 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.12 M CsCl 12 % w/v PEG 3350, 12 % v/v DMSO |