5QHN
PanDDA analysis group deposition of models with modelled events (e.g. bound ligands) -- Crystal Structure of human FAM83B in complex with FMOPL000622a
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-08-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.91587 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 50.541, 50.541, 153.152 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.540 - 1.730 |
| R-factor | 0.1895 |
| Rwork | 0.187 |
| R-free | 0.22650 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 5lzk |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.151 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.510 | 50.510 | 1.770 |
| High resolution limit [Å] | 1.730 | 7.740 | 1.730 |
| Rmerge | 0.094 | 0.026 | 3.003 |
| Rmeas | 0.100 | 0.028 | 3.216 |
| Rpim | 0.033 | 0.010 | 1.113 |
| Total number of observations | 174727 | 2170 | 11273 |
| Number of reflections | 20449 | ||
| <I/σ(I)> | 11.2 | 46.3 | 1 |
| Completeness [%] | 95.5 | 90.2 | 97.7 |
| Redundancy | 8.5 | 8.1 | 7.5 |
| CC(1/2) | 0.999 | 0.999 | 0.497 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 27.3% Tacsimate pH 7.0, 0.15 M NaCl |
