5P21
REFINED CRYSTAL STRUCTURE OF THE TRIPHOSPHATE CONFORMATION OF H-RAS P21 AT 1.35 ANGSTROMS RESOLUTION: IMPLICATIONS FOR THE MECHANISM OF GTP HYDROLYSIS
Experimental procedure
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 40.300, 40.300, 162.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 6.000 - 1.350 |
| Rwork | 0.196 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.700 |
| Refinement software | X-PLOR |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 3-4 (mg/ml) | |
| 2 | 1 | 1 | Tris-HCl | 64 (mM) | |
| 3 | 1 | 1 | dithioerithritol | 1 (mM) | |
| 4 | 1 | 1 | 10 (mM) | ||
| 5 | 1 | 1 | sodium azide | 1 (mM) | |
| 6 | 1 | 1 | PEG1450 | 20 (%(w/v)) |
